УДК: 57.086.8

Using digital off-axis holograms to investigate changes of state of living neuronal cultures

Rybnikov, A.I., Dudenkova, V.V., Muravieva, M.S., Zakharov, Y.N.

Publication in Journal of Optical Technology

For Russian citation (Opticheskii Zhurnal):

Рыбников А.И., Дуденкова В.В., Муравьева М.С., Захаров Ю.Н. Применение цифровых внеосевых голограмм для исследования изменений состояния живых нейронных культур // Оптический журнал. 2013. Т. 80. № 7. С. 66–73.

Rybnikov A.I., Dudenkova V.V., Muravieva M.S., Zakharov Yu.N. Using digital off-axis holograms to investigate changes of state of living neuronal cultures [in Russian] // Opticheskii Zhurnal. 2013. V. 80. № 7. P. 66–73.

For citation (Journal of Optical Technology):

A. I. Rybnikov, V. V. Dudenkova, M. S. Murav’eva, and Yu. N. Zakharov, "Using digital off-axis holograms to investigate changes of state of living neuronal cultures," Journal of Optical Technology. 80(7), 457-462 (2013). https://doi.org/10.1364/JOT.80.000457

Abstract:

Apparatus has been developed and assembled to obtain holograms of a living cell culture by methods of digital and analog holography. The setup demonstrates the advantages of both methods and provides convenient adjustment and monitoring of its parameters for recording a series of digital holograms. Software processing of the results makes it possible to obtain information concerning the total optical thickness of the test samples and to visualize and measure changes in living neuronal cultures. Variations of the structure and manifestations of vital activity of a primary culture of brain cells have been investigated and compared.

Keywords:

digital holography, holographic interferometry, primary cell cultures

Acknowledgements:

The authors express gratitude to scientific fellow E. V. Mitroshina and Professor I. V. Mukhina (Nizhniı˘ Novgorod State Medical Academy) for providing the biological materials, participating in the experiment, and discussing the results.
This study was carried out with the support of the Ministry of Education and Science of the Russian Federation, Contract 8055.

OCIS codes: 090.1995, 090.2880

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