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УДК: 544.164, 543.422.3, 543.426, 547.7, 547.6, 577.323
Biological test methods based on fluorometric genome analysis
Full text «Opticheskii Zhurnal»
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Publication in Journal of Optical Technology
Сибирцев В.С. Методики биотестирования на основе флуорометрического геномного анализа // Оптический журнал. 2017. Т. 84. № 11. С. 84–89.
Sibirtsev V.S. Biological test methods based on fluorometric genome analysis [in Russian] // Opticheskii Zhurnal. 2017. V. 84. № 11. P. 84–89.
V. S. Sibirtsev, "Biological test methods based on fluorometric genome analysis," Journal of Optical Technology. 84(11), 787-791 (2017). https://doi.org/10.1364/JOT.84.000787
This paper presents a generalized spectrofluorescence analysis scheme that makes it possible to estimate not only the quantitative DNA concentration in a sample, but also the variation in its primary and spatial structure by using a system of several specially chosen fluorophores. The paper discusses ways of using this system to rapidly estimate the degree of microbiological contamination of samples and the individual radiosensitivity of living organisms, as well as the genotoxicity of various products, drugs, environmental factors, etc. (including when they act jointly).
fluorescent DNA analysis, fluorophores in DNA, biotesting
OCIS codes: 170.4580, 170.1530, 170.6280, 300.6390, 300.6280, 300.6550, 300.1030
References:1. S. D. Ivanov, L. I. Korytova, V. A. Yamshanov, N. V. Ilyn, and V. S. Sibirtsev, “Leukopenia prognosis by radiation therapy of patients with Hodgkin’s disease,” J. Exp. Clin. Cancer Res. 16(2), 183–188 (1997).
2. S. D. Ivanov, L. I. Korytova, V. A. Yamshanov, N. V. Ilyn, and V. S. Sibirtsev, “Leukopenia prognosis during radiation therapy in patients with Hodgkin’s disease,” J. Exp. Clin. Cancer Res. 16(4), 413–418 (1997).
3. V. S. Sibirtsev, “Analysis of benzo[a]pyrene deactivation mechanisms in rats,” Biochemistry 71(1), 90–98 (2006).
4. A. I. Markosyan, Kh. S. Akalyan, F. G. Arsenyan, R. S. Sukasyan, and B. T. Garibdzhanyan, “Synthesis and biological properties of 3-phenyl- and 3-phenethyl-5-methyl-5-ethyl-4-oxo-3,4,5,6-tetrahydro-benzo[h]quinazolines,” Pharm. Chem. J. 42(6), 313–318 (2008).
5. É. R. Shakurova, T. I. Parfenova, R. Sh. Sufiyarova, A. Z. Khalilova, V. R. Akhmetova, and S. A. Bashkatov, “Synthesis and anti-inflammatory activity of acyl derivatives of taraxasterol,” Pharm. Chem. J. 42(6), 319–321 (2008).
6. V. V. Belakhov and Yu. D. Shenin, “Synthesis and antifungal activity of N-trialkylsilyl derivatives of nystatin,” Pharm. Chem. J. 42(6), 322–325 (2008).
7. O. A. Raevskii, A. N. Razdol’skii, V. D. Tonkopii, I. V. Iofina, and A. O. Zagrebin, “Classificatory and quantitative models of the relationship between the structures of chemical compounds and their toxicity for Daphnia magna,” Pharm. Chem. J. 42(6), 329–334 (2008).
8. O. A. Raevskii, A. N. Razdolskii, Ya. V. Liplavskii, O. E. Raevskaya, and A. V. Yarkov, “Acute toxicity evaluation upon intravenous injection into mice: interspecies correlations, lipophilicity parameters, and physicochemical descriptors,” Pharm. Chem. J. 46(2), 69–74 (2012).
9. A. N. Mironov, ed., Handbook on Carrying Out Preclinical Studies of Medications (Grif i K, Moscow, 2012).
10. A. V. Velikorodov, V. A. Ionova, O. V. Degtyarev, and L. T. Suxenko, “Synthesis and antimicrobial and antifungal activity of carbamate-functionized spiro compounds,” Pharm. Chem. J. 47(12), 715–719 (2013).
11. A. I. Vorob’eva, G. R. Sultanova, A. K. Bulgakov, V. I. Zajnchkovski, and S. V. Kolesov, “Synthesis and biological properties of copolymers based on N,N-diallyl-N,N-dimethylammonium chloride,” Pharm. Chem. J. 47(11), 653–655 (2013).
12. L. V. Anikina, Yu. B. Vikharev, Yu. S. Rozhkova, and Yu. V. Schkljaev, “Synthesis and biological activity of 3-spiro[adamantane-2,3′-isoqui-nolines],” Pharm. Chem. J. 47(12), 707–710 (2013).
13. S. A. Kedik, I. V. Sakaeva, Yu. V. Kochkina, D. V. Eremin, A. V. Panov, and V. V. Suslov, “Synthesis and radioprotector activity of N-vinylpyrrolidone and 2-methyl-5-vinyltetrazole copolymers,” Pharm. Chem. J. 47(12), 726–729 (2013).
14. O. V. Surikova, A. G. Mikhailovskii, and T. F. Odegova, “Synthesis and antimicrobial and antifungal activities of 3-substituted 1-cyano-methyl-3,4-dihydroisoquinolinium chlorides,” Pharm. Chem. J. 49(11), 711–713 (2015).
15. L. N. Divaeva, A. I. Klimenko, A. S. Morkovnik, L. N. Fetisov, T. A. Kuz’menko, A. A. Zubenko, M. A. Bodryakova, and A. N. Bodryakov, “Synthesis and antimicrobial and protistocidal activity of 1-(2-aryloxyethyl- and 2-halobenzyl)-3-(2-hydroxyethyl)-2-imino-1,3-dihydrobenzimidazolines,” Pharm. Chem. J. 49(2), 91–95 (2015).
16. V. V. Odaryuk, N. I. Burakov, L. V. Kanibolotskaya, A. L. Kanibolotskii, I. D. Odaryuk, N. Yu. Lebedeva, E. N. Poddubnaya, and A. N. Shendrik, “Synthesis and antiradical and antibacterial activity of 4-(3′,4′-dihydroxyphenyl)thiazole derivatives,” Pharm. Chem. J. 49(7), 96–98 (2015).
17. V. S. Sibirtsev, I. A. Naumov, E. E. Kuprina, and R. O. Olekhnovich, “Use of impedance biotesting to assess the actions of pharmaceutical compounds on the growth of microorganisms,” Pharm. Chem. J. 50(7), 481–485 (2016).
18. V. S. Sibirtsev, E. N. Glibin, and S. D. Ivanov, “Variation of spectral properties of actinocin derivatives due to equilibrium transformations,” Russ. J. Org. Chem. 36(12), 1812–1818 (2000).
19. V. S. Sibirtsev, A. Yu. Tolmachev, V. V. Suslov, A. V. Garabadzhiu, and V. F. Traven’, “Dependence of fluorescence properties of compounds from psoralen, angelicin, and carbazole series on the character of their terminal substituents,” Russ. J. Org. Chem. 39(6), 881–889 (2003).
20. V. S. Sibirtsev, “Investigation of mechanisms of change in spectral properties during interaction of benzazole, indole, and phenanthridium compounds with DNA,” J. Opt. Technol. 84(5), 294–301 (2017) [Opt. Zh. 84(5), 13–21 (2017)].