Using Raman spectroscopy for the evaluation of extracellular matrices based on heart valves

Timchenko, E.V., Timchenko, P.E., Volova, L.T., Pershutkina, S.V., Shalkovskaya, P.Y.

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For Russian citation (Opticheskii Zhurnal):

Тимченко Е.В., Тимченко П.Е., Волова Л.Т., Першуткина С.В., Шалковская П.Ю. Использование метода спектроскопии комбинационного рассеяния в оценке экстраклеточных матриксов на основе клапанов сердца // Оптический журнал. 2018. Т. 85. № 2. С. 15–19. http://doi.org/10.17586/1023-5086-2018-85-02-15-19

Timchenko E.V., Timchenko P.E., Volova L.T., Pershutkina S.V., Shalkovskaya P.Yu. Using Raman spectroscopy for the evaluation of extracellular matrices based on heart valves [in Russian] // Opticheskii Zhurnal. 2018. V. 85. № 2. P. 15–19. http://doi.org/10.17586/1023-5086-2018-85-02-15-19

For citation (Journal of Optical Technology):

E. V. Timchenko, P. E. Timchenko, L. T. Volova, S. V. Pershutkina, and P. Yu. Shalkovskaya, "Using Raman spectroscopy for the evaluation of extracellular matrices based on heart valves," Journal of Optical Technology. 85(2), 73-76 (2018). https://doi.org/10.1364/JOT.85.000073

Abstract:

Experimental data on the estimation of the qualitative composition of heart valves and their tissue bio-matrices via Raman scattering spectroscopy are presented. The analysis of the Raman spectra allowed identification of the main bands corresponding to collagen, glycosaminoglycans, lipids, and DNA. A two-dimensional analysis of the introduced optical coefficients was performed to compare the compositions of the principal components before and after sample preparation.

Keywords:

Raman spectroscopy, extracellular matrice, heart valve, decellularization

Acknowledgements:

The research was supported by the Ministry of Education and Science of the Russian Federation (Minobrnauka).

OCIS codes: 170.5660, 170.4580, 170.6510

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