УДК: 621.384.4

Effect of ultraviolet radiation from a pulsed ArF laser on the viability of microfungi

Kirtsideli, I.Y., Parfenov, V.A., Zvereva, G.N., Petrov, A.A., Grigorieva, N.O.

Publication in Journal of Optical Technology

For Russian citation (Opticheskii Zhurnal):

Кирцидели И.Ю., Парфенов В.А., Зверева Г.Н., Петров А.А., Григорьева Н.О. Воздействие ультрафиолетового излучения импульсного ArF-лазера на жизнеспособность микроскопических грибов // Оптический журнал. 2017. Т. 84. № 9. С. 19–24.

Kirtsideli I.Yu., Parfenov V.A., Zvereva G.N., Petrov A.A., Grigorieva N.O. Effect of ultraviolet radiation from a pulsed ArF laser on the viability of microfungi [in Russian] // Opticheskii Zhurnal. 2017. V. 84. № 9. P. 19–24.

For citation (Journal of Optical Technology):

I. Yu. Kirtsideli, V. A. Parfenov, G. N. Zvereva, A. Petrov, and N. O. Grigor’eva, "Effect of ultraviolet radiation from a pulsed ArF laser on the viability of microfungi," Journal of Optical Technology. 84(9), 593-597 (2017). https://doi.org/10.1364/JOT.84.000593

Abstract:

The effect of ultraviolet radiation from a pulsed excimer argon fluoride (ArF) laser on the viability of microfungi spores grown in polar latitudes was studied at a radiation wavelength of 193 nm and pulse width of 15 ns. The radiant exposures required to cause the inactivation of spore monolayers of various micromycete species were determined. The viability of the irradiated microfungi spores was found to be affected by the species composition, presence of melanin in the cell wall, and the age of the micromycete culture. Changes in the surface structure of the spores during laser processing were detected via atomic force microscopy. The results of this work indicate the existence of two parallel mechanisms that result in the destruction of spores—namely, a photochemical and a photothermal process—with the latter mechanism playing the predominant role.

Keywords:

microfungi, micromycete, spores, UV radiation, excimer ArF laser, culture methods, atomic force microscope

Acknowledgements:

This work was partially performed using equipment provided by the Center for Cellular and Molecular Technologies of the study of plants and animals and within the framework of the state task specified by the Botanical Institute of the RAS and their thematic plan (01201255604) and the RAS program, supported by the RSF (14-12-00351). The equipment used to conduct the research study was provided with the support of a grant for the leading universities of the Russian Federation (074-U01). The authors are grateful to A. Yu. Polushkin and A. I. Wangonen, employees of the S. I. Vavilov State Optical Institute, as well as to D. A. Palandzhyan, a graduate student of ITMO University, for their help in conducting the experiments.

OCIS codes: 170.1530, 170.1420

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