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DOI: 10.17586/1023-5086-2024-91-08-75-88
УДК: 535.361.22:577.3
Ex vivo determination of perfusion-kinetic parameters of ovaries with granulosa cell carcinoma using reflectance spectroscopy
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Селифонов А.А., Рыхлов А.С., Селифонова Е.И., Тучин В.В. Еx vivo определение перфузионно-кинетических параметров яичников при гранулёзоклеточной опухоли методом отражательной спектроскопии // Оптический журнал. 2024. Т. 91. № 8. С. 75–88. http://doi.org/10.17586/1023-5086-2024-91-08-75-88
Selifonov A.A., Rykhlov A.S., Selifonova E.I., Tuchin V.V. Ex vivo determination of perfusion-kinetic parameters of ovaries with granulosa cell carcinoma using reflectance spectroscopy [in Russian] // Opticheskii Zhurnal. 2024. V. 91. № 8. P. 75–88. http://doi.org/10.17586/1023-5086-2024-91-08-75-88
Subject of study. This study examined the optical properties of healthy and cancerous feline ovarian tissues under the influence of 99.5% glycerol, one of the effective hyperosmotic agents. Aim of study. Determination of perfusion-kinetic parameters of healthy and cancerous ovarian tissues under the influence of 99.5% glycerol. Method. The optical properties of the studied samples were studied by diffuse optical spectroscopy of transmittance and reflectance in a wide spectral range from ultraviolet to near infrared region. Main results. The diffuse reflectance and total transmittance spectra of the studied samples were measured. Using the free diffusion model and Fick’s molecular diffusion equation, and the modified Bouguer–Beer–Lambert law for attenuation of radiation, the diffusion coefficient of interstitial water flux induced by the action of glycerol in ovarian tissue was calculated. The tissue diffusion coefficient of water for healthy ovaries was found as D= (2.2 ± 0.5)х10–6 cm2/s and for ovaries with granulosa cell cancer D = (2.6 ± 0.6)х10–6 cm2/s. Using the obtained diffusion coefficients, we determined the time of complete dehydration of the studied samples under the influence of glycerol. The time for complete dehydration of sections of healthy ovaries in the luteal phase was 19.4 minutes, and for a granulosa cell tumor — 16.6 minutes. Practical significance. The data obtained can be used to assess the overall dehydration of the ovaries when using concentrated glycerol, as well as to develop protocols for drug delivery and organ cryopreservation. The demonstrated effect of optical clearing can be used in therapeutic and diagnostic clinical applications to study molecular structures deep in tissues. Optical clearing technology using hyperosmotic agents, in particular glycerol, leading to improved tissue transparency in the ultraviolet range, can be used in ultraviolet biomedical spectroscopy and for phototherapy.
ovaries, ovarian cancer, granulosa cell carcinoma, glycerol, total transmittance spectra, diffuse reflectance spectra, diffusion coefficient, optical clearing efficiency
Acknowledgements:work was supported by the Russian Science Foundation Grant № 22-75-00021.
OCIS codes: 300.0300
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