Optimizing a high-sensitivity laser fluorescence method

Козулин Р.А. Оптимизация высокочувствительного лазерного флуоресцентного метода // Оптический журнал. 2008. Т. 75. № 5. С. 3–7.

Kozulin R.A. Optimizing a high-sensitivity laser fluorescence method [in Russian] // Opticheskii Zhurnal. 2008. V. 75. № 5. P. 3–7.

R. A. Kozulin, "Optimizing a high-sensitivity laser fluorescence method," Journal of Optical Technology. 75 (5), 285-288 (2008). https://doi.org/10.1364/JOT.75.000285

This paper discusses the process of photodecay of fluorescein as it electrophoretically moves in a thin quartz capillary under the action of laser radiation. It is shown that the photodecay of dyes reduces the measurement sensitivity and imposes a limitation on the power of the exciting radiation.