УДК: 621.391.837.681.3]: [621 + 681: 723

Spectrometer for fluorescence–reflection biomedical research

Kang, Uk, Papayan, G.V., Berezin, V.B., Petrishchev, N.N., Galagudza, M.M.

Publication in Journal of Optical Technology

For Russian citation (Opticheskii Zhurnal):

Kang Uk, Папаян Г.В., Березин В.Б., Петрищев Н.Н., Галагудза М.М. Спектрометр для флуоресцентно-отражательных биомедицинских исследований // Оптический журнал. 2013. Т. 80. № 1. С. 56–67.

Kang Uk, Papayan G.V., Berezin V.B., Petrishchev N.N., Galagudza M.M. Spectrometer for fluorescence–reflection biomedical research [in Russian] // Opticheskii Zhurnal. 2013. V. 80. № 1. P. 56–67.

For citation (Journal of Optical Technology):

Kang Uk, V. B. Berezin, G. V. Papayan, N. N. Petrishchev, and M. M. Galagudza, "Spectrometer for fluorescence–reflection biomedical research," Journal of Optical Technology. 80(1), 40-48 (2013). https://doi.org/10.1364/JOT.80.000040

Abstract:

A fluorescence–reflection multichannel fiber spectrometer has been developed that makes it possible to carry out a wide range of biomedical research, including estimating the concentration of glycation end-products in the skin and the oxidation–reduction state of various tissues. It has the feature of being able to simultaneously record diffuse reflection spectra and fluorescence emission. The fluorescence is excited by an LED that emits at 365 nm. The fluorescence spectra are recorded in the 400–750 nm region, while the reflection spectra are recorded in the 350–750 nm region. The convergence of the results of the measurements is 1.6%. A technique is proposed for correcting the autofluorescence index of the skin that makes it possible to sharply reduce its dependence on the degree of skin pigmentation. The possibilities of the device are illustrated by examples of biomedical research.

Keywords:

fluorescence spectroscopy, reflection spectroscopy, fluorescence diagnostics, autofluorescence

Acknowledgements:

The authors are grateful to Professor A. M. Esayan, Professor E. N. Grineva, E. A. Trofimova, M. V. Vadyukhina, and A. N. Nimgirova for organizing and participating in clinical research on estimating AGEs in the norm, with diabetes, and with renal failure. The authors also express gratitude to the Seoul City Government and the Seoul Institute of Development for supporting this work as part of the international program “Inviting & Supporting Project of Global Leading Institutions” (funds WR100001 for Russian Science Seoul).

OCIS codes: 170.0170, 170.6280, 170.6510, 170.3880, 170.3880, 170.1610, 170.4580

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