A new approach to photodynamic therapy of model abscess in laboratory animals

Tuchina E.S., Musaelyan A.G., Genin V.D., Napsheva A.M., Alipov V.V., Gyulkhandanyan G.V., Tuchin, V.V.

For Russian citation (Opticheskii Zhurnal):

Тучина Е.С., Мусаелян А.Г., Генин В.Д., Напшева А.М., Алипов В.В., Гюльханданян Г.В., Тучин В.В. Новый подход к фотодинамической терапии моделированного абсцесса у лабораторных животных // Оптический журнал. 2026. Т. 93. № 8. С. 84–100. http://doi.org/10.17586/1023-5086-2026-93-08-84-100

Tuchina E.S., Musaelyan A.G., Genin V.D., Napsheva A.M., Alipov V.V., Gyulkhandanyan G.V., Tuchin V.V. A new approach to photodynamic therapy of model abscess in laboratory animals [in Russian] // Opticheskii Zhurnal. 2026. V. 93. № 8. P. 84–100. http://doi.org/10.17586/1023-5086-2026-93-08-84-100

For citation (Journal of Optical Technology):

Abstract:

Subject of the study. The technology of optical skin clearing was used for the first time to increase the efficiency of antimicrobial photodynamic therapy of modeled purulent abscesses in laboratory animals. The aim of the work is to comprehensively analyze the changes occurring in the body of laboratory animals with a modeled purulent abscess during antimicrobial photodynamic therapy using blue LED (428 nm) radiation, pyridyl porphyrin and the technology of optical clearing of biological tissues. Methods. An original method for modeling subcutaneous purulent abscess, a method of photodynamic action using a LED radiation source (428 nm, 7,7 mW/cm²) and 0.1% zinc-meso-tetrakis [3-N-butyl pyridyl] porphyrin, a method of diffuse reflectance spectroscopy for monitoring the optical parameters of the skin in the area of action, ultrasound examination of the abscess cavity volumes, histological methods. Main results. It has been shown that treating the skin of laboratory animals over an abscess with an optical clearing agent (glycerol (70%), DMSO (5%), and water (25%)) increases the penetrating power of LED radiation and enhances the effectiveness of antimicrobial photodynamic therapy by 24–80%, leading to a decrease in the bacterial load from 8 to 0.5 lgCFU/ml and a reduction in the volume of the simulated abscess cavity by 1.8 cm³. Practical significance. The study demonstrates the high efficacy of antimicrobial photodynamic therapy when implementing optical tissue clearing technology in the treatment of simulated subcutaneous purulent abscesses and opens new prospects for the development of minimally invasive methods for the treatment of purulent complications.

Keywords:

antibacterial photodynamic therapy, blue LED radiation, 428 nm, purulent abscess, tissue optical clearing, optical clearing agents

Acknowledgements:

the study was carried out with the financial support of the Russian Science Foundation, Project № 25-24-00370 dated 12/28/2024.

OCIS codes: 170.5180, 170.3660, 170.6510, 170.1610

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