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ISSN: 1023-5086

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ISSN: 1023-5086

Scientific and technical

Opticheskii Zhurnal

A full-text English translation of the journal is published by Optica Publishing Group under the title “Journal of Optical Technology”

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DOI: 10.17586/1023-5086-2023-90-12-61-72

УДК: 004.932.2, 53.082.534

Calculation of blood flow parameters in zebrafish larvae using the phase correlation method

Volkov, M.V., Margaryants, N.B., Myalitsin, D.I., Potemkin, A.V., Guryleva, A.V.

Full text on elibrary.ru

Publication in Journal of Optical Technology

For Russian citation (Opticheskii Zhurnal):

Волков М.В., Маргарянц Н.Б., Мялицин Д.И., Потёмкин А.В., Гурылева А.В. Вычисление параметров кровотока личинки zebrafish с применением метода фазовой корреляции // Оптический журнал. 2023. Т. 90. № 12. С. 61–72. http://doi.org/10.17586/1023-5086-2023-90-12-61-72

 

Volkov M.V., Margaryants N.B., Myalitsin D.I., Potemkin A.V., Guryleva A.V. Calculation of blood flow parameters in zebrafish larvae using the phase correlation method [In Russian] // Opticheskii Zhurnal. 2023. V. 90. № 12. P. 61-72. http://doi.org/10.17586/1023-5086-2023-90-12-61-72

For citation (Journal of Optical Technology):
-
Abstract:

Subject of the study. The parameters of the circulatory system of zebrafish model organisms in the larval stage were investigated. Aim of study. Evaluation of blood flow velocity in vessels of model organisms — zebrafish (danio rerio) larvae using phase correlation methods when analyzing the series of digital microscopy images. Methods. Non-invasive methods of digital microscopy are employed in zebrafish research tasks, enabling high-speed registration of a series of blood flow images. The paper explores novel algorithms for processing the registered images providing the calculation of blood flow parameters based on the phase correlation procedure. These algorithms involve the local matching of images with multiple reference frames, the creation of a synthesized vessel map, the determination of the trajectory of blood flow elements within the selected vessel, and the evaluation of blood flow velocity. Main results. A modified algorithm designed to estimate and compensate for local displacements in zebrafish images using multiple reference frames has been developed. The algorithms based on the phase correlation method for calculation the synthesized map of zebrafish larval blood vessels, along with the trajectories and blood flow velocities within these vessels were developed. These algorithms underwent verification through the processing of digital microscopy data from multiple zebrafish specimens. As a result of this verification vessel maps, blood flow trajectories, and local blood flow velocity values for both arterial and venous vessels were obtained. Practical significance. The study introduces new algorithms for digital microscopy data processing to calculate the blood circulation system parameters of model organisms, such as zebrafish. The developed approaches can be used to study blood flow in tasks related to medicines testing or genetic studies.

Keywords:

zebrafish, danio-rerio, digital microscopy, videocapillaroscopy, blood flow velocity, image matching, Fourier transform, phase correlation

Acknowledgements:

the work was carried out with the financial support of the RSF grant 22-49-08012.

OCIS codes: 100.0100, 170.0180, 180.0180

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