Quantitative determination of the calcium-ion concentration from fluorescence measurements of single-wavelength dyes by means of laser-scanning microscopy

Захаров Ю.Н., Ершова А.В. Количественное определение содержания ионов кальция по измерениям флуоресценции однодлинноволновых красителей с помощью лазерной сканирующей микроскопии // Оптический журнал. 2011. Т. 78. № 10. С. 36–37.

Zakharov Yu.N., Ershova A.V. Quantitative determination of the calcium-ion concentration from fluorescence measurements of single-wavelength dyes by means of laser-scanning microscopy [in Russian] // Opticheskii Zhurnal. 2011. V. 78. № 10. P. 36–37.

Yu. N. Zakharov and A. V. Ershova, "Quantitative determination of the calcium-ion concentration from fluorescence measurements of single-wavelength dyes by means of laser-scanning microscopy," Journal of Optical Technology. 78(10), 664-665 (2011). https://doi.org/10.1364/JOT.78.000664

This paper discusses the problem of obtaining reliable quantitative experimental data when the functional activity of brain cells is studied by fluorescence microscopy, using specific calcium dyes. A method is developed for determining the calcium-ion concentration from the current fluorescence intensity at a single wavelength, starting from the coloration protocol and the excitation conditions and making it unnecessary to carry out calibration in each experiment.