ITMO
ru/ ru

ISSN: 1023-5086

ru/

ISSN: 1023-5086

Scientific and technical

Opticheskii Zhurnal

A full-text English translation of the journal is published by Optica Publishing Group under the title “Journal of Optical Technology”

Article submission Подать статью
Больше информации Back

УДК: 53.086 + 577.3

Fluorescence analysis of the metabolic activity patterns of a neuronal–glial network

For Russian citation (Opticheskii Zhurnal):

Захаров Ю.Н., Митрошина Е.В., Ведунова М.В., Коротченко С.А., Калинцева Я.И., Потанина А.В., Мухина И.В. Флуоресцентный анализ паттернов метаболической активности нейрон-глиальной сети // Оптический журнал. 2012. Т. 79. № 6. С. 47–51.

 

Zakharov Yu. N., Mitroshina E. V., Vedunova M. V., Korotchenko S. A., Kalintseva Ya. I., Mukhina I. V., Potanina A. V. Fluorescence analysis of the metabolic activity patterns of a neuronal–glial network [in Russian] // Opticheskii Zhurnal. 2012. V. 79. № 6. P. 47–51.

For citation (Journal of Optical Technology):

Yu. N. Zakharov, E. V. Mitroshina, M. V. Vedunova, S. A. Korotchenko, Ya. I. Kalintseva, I. V. Mukhina, and A. V. Potanina, "Fluorescence analysis of the metabolic activity patterns of a neuronal–glial network," Journal of Optical Technology. 79(6), 348-351 (2012). https://doi.org/10.1364/JOT.79.000348

Abstract:

This paper shows that spontaneous network activity is present in a neuronal–glial network in the form of patterns that have the same character of the oscillations and synchronous switching of the generation regimes of all the patterns by recording the fluorescence of specific indicators for visualizing the dynamics of the calcium oscillations in cell cultures and culture sections of the hippocampus of the brain.

Keywords:

optics for medicine and biotechnology, imaging system, confocal microscopy, neurophysiology, neuron, glia

OCIS codes: 170.0170, 170.0110, 170.1790, 170.2520, 170.5810, 170.5380, 170.1530

References:

1. I. V. Mukhina, V. B. Kazantsev, L. G. Khaspekov, Yu. N. Zakharov, M. V. Vedunova, E. V. Mitroshina, S. A. Korotchenko, and E. A. Koryagina, “Multielectrode arrays—new possibilities in the study of the plasticity of a neural network,” Sovrem. Tekhnol. Med. No. 1, 8 (2009).
2. Ya. I. Kalintseva, A. V. Potanina, A. S. Pimashkin, I. V. Mukhina, V. B. Kazantsev, Yu. N. Zakharov, and A. V. Sem’yanov, “Spontaneous and glutamate-induced calcium astrocyte activity in culture sections of the rat hippocampus,” Vesnt. Mosk. Univ., Ser. 16: Biol. No. 2, 13 (2011).
3. M. Paredes, J. C. Etzler, L. T. Watts, W. Zheng, and J. D. Lechleiter, “Chemical calcium indicators,” Methods 46, 143 (2008).
4. A. Nimmerjahn, F. Kirchhoff, J. N. Kerr, and F. Heimchen, “Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo,” Nat. Methods 1, 31 (2004).
5. A. S. Pimashkin, A. A. Lebedinski˘ı, and A. V. Sem’yanov, “Using mathematical models to determine the reliability of the differences of the parameters in dynamic biological systems,” Vestn. NNGU. Biol. No. 2, 51 (2010).
6. S. J. Mulligan and B. A. MacViar, “Monitoring intracellular Ca2+ in brain slices with fluorescent indicators,” in Voltage-Gated Calcium Channels (Eurecan.com and Kluwer Academic/Plenum Publishers, 2005).