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ISSN: 1023-5086


ISSN: 1023-5086

Scientific and technical

Opticheskii Zhurnal

A full-text English translation of the journal is published by Optica Publishing Group under the title “Journal of Optical Technology”

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DOI: 10.17586/1023-5086-2020-87-03-37-45

УДК: 535.375.5

Optical analysis of bone tissue by Raman spectroscopy in experimental osteoporosis and its correction using allogeneic hydroxyapatite

For Russian citation (Opticheskii Zhurnal):

Тимченко Е.В., Тимченко П.Е., Писарева Е.В., Власов М.Ю., Волова Л.Т., Федотов А.А., Федорова Я.В., Тюмченкова А.С., Романова Д.А., Даниэль М.А., Субатович А.Н. Оптический анализ костной ткани методом спектроскопии комбинационного рассеяния при экспериментальном остеопорозе и его коррекции с помощью аллогенного гидроксиапатита // Оптический журнал. 2020. Т. 87. № 3. С. 37–45.  

Timchenko E.V., Timchenko P.E., Pisareva E.V., Vlasov M.Yu., Volova L.T., Fedotov A.A., Fedorova Ya.V., Tyumchenkova A.S., Romanova D.A., Daniel M.A., Subatovich A.N. Optical analysis of bone tissue by Raman spectroscopy in experimental osteoporosis and its correction using allogeneic hydroxyapatite [in Russian] // Opticheskii Zhurnal. 2020. V. 87. № 3. P. 37–45.

For citation (Journal of Optical Technology):

E. V. Timchenko, P. E. Timchenko, E. V. Pisareva, M. Yu. Vlasov, L. T. Volova, A. A. Fedotov, Ya. V. Fedorova, A. S. Tyumchenkova, D. A. Romanova, M. A. Daniel, and A. N. Subatovich, "Optical analysis of bone tissue by Raman spectroscopy in experimental osteoporosis and its correction using allogeneic hydroxyapatite," Journal of Optical Technology. 87(3), 161-167 (2020).


The results of experimental studies of bone tissue in models of osteoporosis are presented, and the effectiveness of the correction of this pathological condition using allogeneic hydroxyapatite is evaluated by Raman spectroscopy. During the studies, Raman spectra of the cancellous and cortical bone tissues were obtained and the spectra were deconvolved into bands using the Gaussian function. The introduced coefficients make it possible to evaluate the effectiveness of various doses of hydroxyapatite (10, 20, 30 mg/kg) for the correction of osteoresorption in models of osteoporosis by ovariectomy. It was established that, in contrast to the cortical bone, pronounced changes in the coefficients I1069/I1739, I850/I1739, and I1243/I1739 were not observed in the cancellous bone. The introduction of a suspension of hydroxyapatite showed that during the indicated treatment period, the consequences of ovariectomy can be reversed by 40%–60% for the cancellous bone and almost completely for the cortical bone (70%–100%).


Raman spectroscopy, optical coefficients, osteoporosis, hydroxyapatite, ovariectomy, collagen matrice, spectrum deconvolution


The research was supported by the Russian Foundation for Basic Research (RFBR) (18-315-20017\18).

OCIS codes: 170.5660, 170.4580


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