Analytical method of sequencing DNA using spectral fluorescence markers

Беленький Б.Г., Козулин Р.А., Курочкин В.Е., Золотарев В.М. Аналитический метод определения последовательности ДНК (секвенирование) по спектральным флуоресцентным меткам // Оптический журнал. 2003. Т.70. №1. С. 65–68.

Belen’kii B. G., Kozulin R. A., Kurochkin V. E., Zolotarev V. M. Analytical method of sequencing DNA using spectral fluorescence markers [in Russian] // Opticheskii Zhurnal. 2003. V. 70. No 1. P. 65–68.

B. G. Belen’kii, V. E. Kurochkin, R. A. Kozulin, and V. M. Zolotarev, "Analytical method of sequencing DNA using spectral fluorescence markers," Journal of Optical Technology. 70(1), 55-57 (2003). https://doi.org/10.1364/JOT.70.000055

This paper discusses the physical bases for analyzing the nucleotide sequence of a DNA molecule by means of capillary electrophoresis and possible ways of increasing the throughput of the method. An analysis is presented of schematic diagrams for the detection of nucleotides by means of laser excitation of the luminescence of primers attached to nucleotides from the spectral fluorescence of the markers.